MEASUREMENT OF OXIDATIVE STRESS
Why is the balance leaning on the wrong side?
Excess pro-oxidants or the body’s inability to defend itself?
We separately evaluate pro-oxidants and antioxidants. Thus by combining the two, it is possible to determine whether the oxidative stress is due to an increase in the production of oxygen radicals or a decrease in the ability to eliminate these oxygen radicals.
- dROMs TEST: This test provides an indication of the general well-being of the body, identifies risks and predisposition to oxidative damage in subjects exposed to risk factors, suffering from diseases or undergoing treatment.
Measurement of reactive forms of oxygen present in serum and plasma. ROMs (Reactive Oxygen Metabolites) are generated by the peroxidation of organic substrates (lipids, proteins, DNA,…). They are relatively more stable than oxygenated radicals, which allows them to be quantified.
- OXY-Adsorbent TEST : This test allows to evaluate the state of the antioxidant defenses and/or the efficiency of specific antioxidant treatments.
Measurement of the capacity to eliminate oxygenated radicals. The test evaluates the capacity of the plasma to oppose the massive oxidative action of an excess of hypochloric acid. By colorimetric reaction, we measure the quantity of hypochloric acid that the antioxidant barrier is able to reduce.
- BAP TEST: This test allows to evaluate the state of antioxidant defenses and/or the effectiveness of specific antioxidant treatments.
It measures the capacity of the blood to convert a Fe3+ reagent into Fe2+. This reduction is generated by an adequate reducing system, i.e. antioxidant, as is the plasma. As ferric ions are already naturally present in our body, the BAP (biological antioxidant potential) test provides a measure of the “physiological” antioxidant reducing capacity of iron. This test measures the chemically active antioxidant capacity (scavengers) of the plasma barrier.
And to go further, it is necessary to dose the primary antioxidants: SODe, GSH-pxe and vitamin E but also to dissociate animals with inflammatory phenomenon by the assay of a marker of inflammation, the SAA for the equine , haptoglobin for cattle ….